The extent of gene essentiality and buffering by duplicates is not conserved across organisms Supplementary Material

Abbreviations: CAI – Codon Adaptation Index; D – effective gene family size (number of additional gene duplicates); E-value – expectation value; KD – knockdown; KO – knockout; MIPS Munich Information Center for Protein Sequences; P(S) – probability of survival upon singleor double-gene KO or KD; R – squared Pearson correlation coefficient; SGA– Synthetic Genetic Array; SSL – synthetic sick or lethal (mutant); SYD – Stanford Yeast Database; WGD – whole-genome duplication Organisms: M. genitalium Mycoplasma genitalium; H. pylori – Helicobacter pylori; H. influenzae – Haemophilus influenzae; M. tuberculosis Mycobacterium tuberculosis; Paer Pseudomonas aeruginosa; B. subtilis – Bacillus subtilis; E. coli – Escherichia coli; S. cerevisiae – Saccharomyces cerevisiae (yeast); C. elegans – Caenorhabditis elegans (worm); D. melanogaster – Drosophila melanogaster (fly); M. musculus – Mus musculus (mouse)